A recent genetic analysis of a Cambodian ox called a kouprey matches fossil evidence that proves Cambodia's national animal is indeed its own species.
The latest study joins a growing body of evidence showing that the kouprey (pronounced "ko-prah") is not a hybrid between two related species of ox, the banteng and zebu, as was previously suggested.
French evolutionary biologists Alexandre Hassanin and Anne Ropiquet at the National Museum of Natural History in Paris, France, sequenced kouprey DNA and compared it to that of related wild and domestic oxen species.
There are two types of DNA in a cell: nuclear DNA and mitochondrial DNA (mtDNA). Nuclear DNA is a combination of maternally and paternally inherited genes, and mtDNA is inherited exclusively from the mother. Hassanin analyzed both types of DNA in the new study.
"These molecular data allow us to study the evolutionary history of both paternal and mitochondrial lineages," he wrote by email from Vietnam.
If the kouprey is a hybrid of its close oxen relatives, its nuclear genes would have been a combination of the two hypothetical parent species. Instead Hassanin found that the kouprey's nuclear sequences differed from those of banteng and zebu.
"Our interpretations are therefore that the kouprey is a real wild species, different from all other wild oxen," wrote Hassanin.
The study will appear in the November issue of the journal Proceedings of the Royal Society B.
Disappearing National Icon
The kouprey, which resembles a dark-coated ox with massive, curling horns, was first recognized as a species in 1937. In 1960 Cambodia made it the national symbol.
But habitat destruction and hunting took its toll, and many experts believe the last scientific observation of the animal in the wild was in 1957.
"I cannot imagine that if there were any kouprey left today we wouldn't be aware of them," said Gary J. Galbreath, an evolutionary biologist at Northwestern University in Evanston, Illinois, who was not involved in the study.
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