DNA tests carried out by a U.S. laboratory prove that remains exhumed last year belong to two children of Tsar Nicholas II, putting to rest questions about what happened to Russia's last royal family, a regional governor said Wednesday.
The bone fragments dug up are those of Crown Prince Alexei and his sister, Maria, whose remains had been missing since the family was murdered in 1918 as Russia descended into civil war, said Eduard Rossel, governor of the Sverdlovsk region (see map of Russia).
"We have now found the entire family," he told reporters in Yekaterinburg, the city where the remains were exhumed about 900 miles east of Moscow.
The confirmation could bring the tortured history of the Russian imperial family closer to closure and end royal supporters' persistent hopes that members of the tsar's immediate family survived the massacre.
Nicholas II abdicated in 1917 as revolutionary fervor swept Russia, and he and his family were detained.
The tsar, his wife, Alexandra, and their son and four daughters were fatally shot on July 17, 1918, in a basement room of the merchant's house where they were being held in Yekaterinburg.
The remains of Nicholas, Alexandra, and three of their daughters were unearthed in 1991 as the Soviet Union was collapsing.
Genetic tests convinced experts of their authenticity and identified one set as those of Anastasia, a daughter some have said survived.
The Russian Orthodox Church canonized Nicholas and his family in 2000, even as it expressed doubts that the remains were indeed those of the tsar's family.
The remains of Alexei and Maria, however, had never been located, leading to decades of speculation that perhaps one or both had survived.
(Watch "Video: Last Tsar's Missing Children Found, Expert Says" [October 1, 2007].)